The ability of the bovine cutaneous papifioma agent

Five different cutaneous papifioma isolates (215, 219, 233-A, 247, and 260) were used for inoculation of the skin and urinary bladder in 59 nonvaccinated and twenty vaccinated calves 2—6mo. of age. Some of the non vaccinated and all of the vaccinated calves were part of a previously reported experiment on immunity (7). Inocula were prepared as a 10 % isotonic saline suspension of bovine wart tissue which had been stored in 50 per cent glycerine at 4°C. Duplicate injections of approximately 0.5 ml of inoculum were made into the submucosa of the urinary bladder either by suprapubic cystostomy or, in female calves, transurethrally with a cystoscope and a No. 28 Stem-McCarthy working element. The inoculum used in the urinary bladder was also inoculated intradermally